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Image Search Results
Journal: Plant Methods
Article Title: Detection of Cucumber green mottle mosaic virus in low-concentration virus-infected seeds by improved one-step pre-amplification RT-qPCR
doi: 10.1186/s13007-022-00901-2
Figure Lengend Snippet: Cucumber green mottle mosaic virus (CGMMV) was detected by improved one-step pre-amplification RT-qPCR. CGMMV-infected single seed sample; CGMMV reference material; simulated CGMMV-contaminated seed powder base material; cucumber mosaic virus (CMV); zucchini yellow mosaic virus (ZYMV); melon yellow spot virus (MYSV). Tests were performed using total RNA extracted from leaves and seeds infected with one of the four viruses studied. For each assay, RNA extracted from uninfected seeds was used as negative control, along with a non-template control (NTC). Amplification plots show the normalized fluorescence values (ΔRn) versus the amplification cycle number, and horizontal lines denote the threshold limit of the test. Samples were tested in duplicate. Amplification plots correspond to improved one-step pre-amplification RT-qPCR
Article Snippet: Positive control samples (C2300) for
Techniques: Virus, Amplification, Quantitative RT-PCR, Infection, Negative Control, Control, Fluorescence